DTU-researchers develop a novel method for rapidly detecting SARS-CoV-2 RNA that may also be designed to detect other diseases.
The current SARS-CoV-2 RNA detection methods recommended by the World Health Organization profoundly rely on the roles of biological enzymes. High cost, stringent transportation and storage conditions, and the global supply shortages of enzymes limit large-scaled testing. This means that most countries have to prioritize testing on vulnerable cases, which delays diagnostics and identification of positive cases, which again can hamper pandemic mitigation and suppression.
Quantitative reverse transcription-polymerase chain reaction(qRT-PCR) is still the gold standard for whole-genome detection and has played a crucial role in controlling the Covid-19 pandemic. However, the sample-to-result takes several hours. The method necessitates a complex thermocycler instrument to raise and lower the reaction temperature in discrete steps.
Simpler and less expensive
Non-enzymatic isothermal amplification methods, being more straightforward and faster, have shown promising potentials to substitute the qRT-PCR. Although these methods perform very well when the target gene is short, they have yet to function efficiently to detect whole genomes (long DNA or RNA targets).
During the COVID-19 pandemic, the euro area alone experienced a 3.8% drop in GDP within the first quarter of 2020 (Eurostat 2020). Thus, developing a lower-cost methodology for pathogen detection would be highly beneficial for both patients and the healthcare systems aiming to battle future pandemics.
Associate Professor Yi Sun and Postdoc Mohsen Mohammadniaei at DTU Health Tech have invented a one-pot assay, which they have named NISDA (Non-enzymatic isothermal strand displacement and amplification assay). The assay is for rapid detection of SARS-CoV-2 RNA without the need for the RNA reverse transcription step of the qRT-PCR methodology. It enables a single-step detection routine. The user only needs to add the sample into a single tube, place it in the instrument and wait for 30 minutes to obtain the result.
The assayworks at a constant temperature requires no enzymes, and is based on the toehold-mediated strand displacement (TMSD) approach. TMSD is an enzyme-free molecular tool from which one strand of DNA or RNA (output) is displaced by another strand (input) to form a more stable duplex structure.
High accuracy and sensitivity
The NISDA assay detected a very low concentration of RNA (10 copies/µL) in only 30 minutes. In collaboration with Hvidovre Hospital and Bispebjerg Hospital, the research team could clinically validate the NISDA assay, representing 100 % specificity and 96.77% and 100% sensitivity when setting up in the laboratory and hospital, respectively.
We exploited the TMSD approach and designed three DNA probes. One probe exchanged the whole genome to a short DNA strand. The other two probes utilized the exchanged short DNA to trigger a fluorescence signal amplification cascade reaction. The beauty of the NISDA assay is its simplicity. We removed the usage of enzymes to reduce the assay cost and enhance its robustness at room temperature.”
Yi Sun, Associate Professor
The extracted RNA from throat swab samples is added to the reaction mixture and incubated at 42 °C for 30 minutes in the assay workflow. The next step is fluorescence measurement, and samples with significantly higher fluorescence signals than the control samples (negative) are considered positive.
Towards a multiple disease diagnostics tool
“Being directly involved in improving people’s health is the ultimate dream of a biomedical researcher. We believe that the NISDA assay has given us this wonderful chance to attain that ambition,” Postdoctoral Researcher Mohsen Mohammadniaei says.
“The next step is to further design the NISDA assay for detecting different pathogens and develop a point-of-care diagnostic device for multiple disease diagnostics.Another advantage of the NISDA assay is its ability to be designed for short RNA targets such as cancer biomarker microRNA. We are currently exploring different schemes for the commercialization of the NISDA assay. We are certain that the NISDA assay will become widely known soon”, Associate Professor Yi Sun finishes.
DTU (Technical University of Denmark)
Mohammadniaei, M., et al. (2021) A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA. Nature Communications. doi.org/10.1038/s41467-021-25387-9.
Posted in: Molecular & Structural Biology | Biochemistry
Tags: Assay, Biomarker, Cancer, Cancer Biomarker, Diagnostic, Diagnostics, DNA, Enzyme, Fluorescence, Gene, Genome, Healthcare, Hospital, Laboratory, MicroRNA, Pandemic, Pathogen, Polymerase, Polymerase Chain Reaction, Research, RNA, SARS, SARS-CoV-2, Throat, Transcription
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